3WZF

Crystal structure of human cytoplasmic aspartate aminotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9
Spacegroup name	P 43 21 2
Unit cell lengths	93.388, 93.388, 107.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.429 - 2.991
R-factor	0.2566
Rwork	0.254
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ajr
RMSD bond length	0.008
RMSD bond angle	1.189
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	2.991	3.000
Number of reflections	10049
Completeness [%]	99.4	100
Redundancy		12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1M Bis-Tris 21%(w/v) PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K