3WYR

Crystal structure of Killer cell immunoglobulin-like receptor 2DL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9546
Spacegroup name	P 4 21 2
Unit cell lengths	87.836, 87.836, 105.996
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.632 - 2.800
R-factor	0.2124
Rwork	0.211
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	KIR2DS4 PDB code 3H8N
RMSD bond length	0.003
RMSD bond angle	0.717
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.632	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.059	0.333
Number of reflections	10753
<I/σ(I)>	10.8	2.2
Completeness [%]	100.0	100
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	16% PEG 3350, 4% tacsimate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K