3WY8
Crystal Structure of Protease Anisep from Arthrobacter Nicotinovorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.017, 103.017, 103.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 1.700 |
| R-factor | 0.15502 |
| Rwork | 0.154 |
| R-free | 0.18103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cp7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.999 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | 0.500 |
| Number of reflections | 20158 | |
| <I/σ(I)> | 44.9 | 7.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.2 | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 4.3M sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
