3WY2
Crystal structure of alpha-glucosidase in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.219, 103.809, 176.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.085 - 1.471 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.17910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.560 |
| High resolution limit [Å] | 1.470 | 4.400 | 1.470 |
| Rmerge | 0.052 | 0.023 | 0.539 |
| Number of reflections | 197034 | ||
| <I/σ(I)> | 25.2 | 73.7 | 3.9 |
| Completeness [%] | 99.8 | 99.5 | 98.9 |
| Redundancy | 7.3 | 6.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 22% PEG 6000, 0.1M HEPES, 0.5M lithium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
