3WXZ
The structure of the I375F mutant of CsyB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.900, 104.750, 73.160 |
Unit cell angles | 90.00, 114.33, 90.00 |
Refinement procedure
Resolution | 41.184 - 2.303 |
R-factor | 0.1755 |
Rwork | 0.173 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wxy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.038 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.053 | 0.268 |
Number of reflections | 42426 | |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 3.8 | 3.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM PIPES-NaOH, 6% PEG 4000, 250mM LiCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |