3WXX
Crystal Structure of a T3SS complex from Aeromonas hydrophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-08 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.662, 151.942, 106.253 |
| Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.21493 |
| Rwork | 0.213 |
| R-free | 0.25222 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.026 | |
| Number of reflections | 48982 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 96.5 | |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 1.1M Sodium Malonate, 0.1M HEPES, 0.5% Jeffamine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
