3WXK
Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.485, 122.139, 154.515 |
| Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.370 |
| R-factor | 0.19317 |
| Rwork | 0.190 |
| R-free | 0.25099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.651 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.043 | 0.474 |
| Number of reflections | 80879 | |
| <I/σ(I)> | 14.6 | 1.7 |
| Completeness [%] | 95.8 | 96 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20-30% PEG 400, 0.1M HEPES, 0.01M MAGNESIUM SULPHATE, 11% 1,6-HEXANEDIOL , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
