3WX4

CRYSTAL STRUCTURE of T4 PHAGE ARN PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-04
Detector	ADSC QUANTUM 315r
Spacegroup name	H 3 2
Unit cell lengths	81.959, 81.959, 149.424
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.000 - 1.900
R-factor	0.208
Rwork	0.208
R-free	0.23200
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.767
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge		0.478
Number of reflections	15419
<I/σ(I)>		6.15
Completeness [%]	99.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		50MM TRIS, 100MM NACL, 3.6M NA- FORMATE(RESERVOIR), 0.1M TRIS(RESERVOIR), PH 8.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K