3WX0
The crystal structure of D-lactate dehydrogenase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 131.029, 131.029, 405.746 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.030 - 3.300 |
| R-factor | 0.23347 |
| Rwork | 0.231 |
| R-free | 0.27116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j49 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.030 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 32128 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100mM sodium acetate (pH 4.5), 0.8M sodium dihydrogenphosphate, 1.2M potassium monohydrogen phosphate., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
