3WWX
Crystal structure of D-stereospecific amidohydrolase from Streptomyces sp. 82F2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-17 |
| Detector | Saturn A200 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.290, 74.681, 76.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.511 - 1.490 |
| R-factor | 0.1419 |
| Rwork | 0.141 |
| R-free | 0.16160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cef |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.315 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.520 |
| High resolution limit [Å] | 1.490 | 4.040 | 1.490 |
| Rmerge | 0.053 | 0.032 | 0.244 |
| Number of reflections | 54112 | ||
| <I/σ(I)> | 20 | ||
| Completeness [%] | 96.4 | 98.6 | 73.5 |
| Redundancy | 5.9 | 6.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 1.5 M Citrate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
