3WWV
C-terminal domain of stomatin operon partner protein 1510-C from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-02-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 75.847, 75.847, 43.631 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.960 - 2.400 |
| R-factor | 0.22464 |
| Rwork | 0.221 |
| R-free | 0.25796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2exd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.316 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0048) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.570 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 | 0.404 |
| Number of reflections | 3165 | |
| <I/σ(I)> | 25.9 | 6.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% Jeffamine M-600, 0.1M HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
