3WWS
Crystal structure of Serine/threonine-protein kinase 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | AREA DETECTOR |
| Collection date | 2011-04-15 |
| Detector | XENTRONICS |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.831, 32.678, 55.830 |
| Unit cell angles | 90.00, 109.63, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.010 |
| R-factor | 0.27108 |
| Rwork | 0.269 |
| R-free | 0.31141 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AutoSol) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.670 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 43771 |
| Completeness [%] | 58.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1M Tris-HCl pH 7.4. 10%(m/v) PEG3350, 0.1M (NH4)3PO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
