3WWL

Crystal structure of lysine biosynthetic amino acid carrier protein LysW from Thermus thermophilus conjugated with alpha-aminoadipate

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2009-10-24
Detector	ADSC QUANTUM 210
Wavelength(s)	1.28105, 1.28323, 1.25720
Spacegroup name	P 32 2 1
Unit cell lengths	48.932, 48.932, 38.654
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.190 - 1.200
R-factor	0.14592
Rwork	0.145
R-free	0.15544
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.507
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	16643
<I/σ(I)>	58.563	11.8
Completeness [%]	97.2	100
Redundancy	10.8	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	2.0M Ammonium sulfate, 0.1M Sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K