3WWK
Crystal structure of CLEC-2 in complex with rhodocytin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.851, 117.072, 152.238 |
| Unit cell angles | 90.00, 115.78, 90.00 |
Refinement procedure
| Resolution | 37.560 - 2.980 |
| R-factor | 0.28407 |
| Rwork | 0.282 |
| R-free | 0.32492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2C6U and 2VRP |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.050 |
| High resolution limit [Å] | 2.980 | 2.983 |
| Number of reflections | 41508 | |
| <I/σ(I)> | 16.9 | 2.9 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 0.1M Hepes (pH 7.6), 0.2M L-proline, 10% (w/v) PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
