3WVW
Crystal structure of RuCO/apo-WTFr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-17 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.863, 181.863, 181.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.430 - 2.000 |
| R-factor | 0.16004 |
| Rwork | 0.158 |
| R-free | 0.19917 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.980 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17978 | |
| <I/σ(I)> | 44.51 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1M ammonium sulfate, 20 mM cadmium sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
