3WVQ
Structure of ATP grasp protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.660, 86.750, 94.260 |
| Unit cell angles | 73.74, 85.93, 68.26 |
Refinement procedure
| Resolution | 45.177 - 1.955 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.23120 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 1.950 | 5.820 | 1.950 |
| Rmerge | 0.116 | 0.038 | 0.619 |
| Rmeas | 0.045 | 0.717 | |
| Number of reflections | 154262 | 11758 | 47097 |
| <I/σ(I)> | 9.94 | 29.96 | 2.31 |
| Completeness [%] | 96.7 | 97.8 | 91.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 0.1M Tris-HCl, pH 8.3, 1.175M Lithium Sulfate, 10mM L-Met, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
