3WVG
Time-Resolved Crystal Structure of HindIII with 0sec soaking
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-01-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.656, 140.583, 96.294 |
Unit cell angles | 90.00, 112.10, 90.00 |
Refinement procedure
Resolution | 39.700 - 2.250 |
R-factor | 0.1797 |
Rwork | 0.177 |
R-free | 0.22620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a4k |
RMSD bond length | 0.016 |
RMSD bond angle | 1.783 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.128 | 0.892 |
Number of reflections | 93316 | |
<I/σ(I)> | 16.5 | 2.2 |
Completeness [%] | 99.7 | 97.74 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10-15% PEG 3350, 300mM NaSCN, 10% Glycerol, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |