3WVC
Guanylylpyridinol (GP)-bound HcgF from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.570, 97.530, 47.120 |
| Unit cell angles | 90.00, 111.17, 90.00 |
Refinement procedure
| Resolution | 48.760 - 2.000 |
| R-factor | 0.16621 |
| Rwork | 0.163 |
| R-free | 0.22973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet-labelled HcgF from Methanocaldococcus jannaschii PDB ENTRY 3WVA |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.976 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 | |
| High resolution limit [Å] | 2.000 | 12.000 | 2.000 |
| Rmerge | 0.051 | 0.037 | 0.124 |
| Number of reflections | 22368 | ||
| <I/σ(I)> | 20.35 | 34.34 | 11.13 |
| Completeness [%] | 99.0 | 97.3 | 98.9 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 40%(v/v)MPD, 200mM lithium sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
