3WVB
HcgF from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.270 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.310, 73.110, 45.530 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 44.280 - 1.700 |
| R-factor | 0.1476 |
| Rwork | 0.145 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet-labelled HcgF PDB ENTRY 3WVA |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.286 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 | |
| High resolution limit [Å] | 1.700 | 12.000 | 1.700 |
| Rmerge | 0.023 | 0.031 | 0.042 |
| Rmeas | 0.036 | 0.050 | |
| Number of reflections | 31585 | 96 | 4855 |
| <I/σ(I)> | 39.88 | 54.08 | 25.62 |
| Completeness [%] | 98.6 | 95 | 96.8 |
| Redundancy | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20%(w/v)PEG3000, 200mM sodium chloride, 100mM HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
