3WUV

Structure basis of inactivating cell abscission with chimera peptide 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	233.066, 134.671, 88.325
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	48.644 - 2.790
R-factor	0.2017
Rwork	0.201
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eir
RMSD bond length	0.006
RMSD bond angle	0.863
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.790	2.800
Number of reflections	67471
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.8M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K