3WUH

Qri7 and AMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 3 2
Unit cell lengths	180.315, 180.315, 180.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.937
R-factor	0.2255
Rwork	0.223
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k25
RMSD bond length	0.002
RMSD bond angle	0.667
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.100
High resolution limit [Å]	2.937
Number of reflections	22014

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG 400, 3% w/v dextran sulfate sodium salt (Mr 5000), 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K