3WUD
X-ray crystal structure of Xenopus laevis galectin-Ib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-11-27 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.210, 35.320, 58.510 |
| Unit cell angles | 90.00, 111.82, 90.00 |
Refinement procedure
| Resolution | 18.920 - 1.680 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25329 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wuc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.152 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.920 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.046 | 0.344 |
| Number of reflections | 13973 | |
| <I/σ(I)> | 14.3 | 2.9 |
| Completeness [%] | 94.8 | 91.4 |
| Redundancy | 4.04 | 4.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 34% PEG 3350, 0.1M MES, 0.2M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
