3WUC
X-ray crystal structure of Xenopus laevis galectin-Va
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-01 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.650, 48.870, 61.090 |
| Unit cell angles | 90.00, 114.08, 90.00 |
Refinement procedure
| Resolution | 37.880 - 1.600 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.21659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzw |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.267 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.880 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.056 | 0.172 |
| Number of reflections | 36200 | |
| <I/σ(I)> | 12.8 | 2.5 |
| Completeness [%] | 98.7 | 96.9 |
| Redundancy | 3.56 | 3.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 2.4M sodium malonate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
