3WU5
Reduced E.coli Lon Proteolytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 86.350, 86.350, 124.375 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.790 - 2.070 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rr9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.072 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.069 | 2.070 |
| Number of reflections | 63282 | |
| <I/σ(I)> | 21.6 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0M AMMONIUM SULFATE, 0.1M BIS-TRIS, 10MM DTT, PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
