3WTR
Crystal structure of E. coli YfcM bound to Co(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.60490 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.289, 36.938, 37.544 |
| Unit cell angles | 90.00, 101.27, 90.00 |
Refinement procedure
| Resolution | 36.820 - 1.960 |
| R-factor | 0.1718 |
| Rwork | 0.170 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pdn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.960 |
| Number of reflections | 12114 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 26% PEG 3350, 180mM ammonium sulfate, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
