3WSW
Crystal structure of minor L-lactate dehydrogenase from Enterococcus mundtii in the ligands-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-31 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.770, 123.540, 85.790 |
| Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
| Resolution | 29.670 - 2.300 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ldn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | 0.413 |
| Number of reflections | 57963 | |
| <I/σ(I)> | 8.2 | 2.4 |
| Completeness [%] | 97.3 | 97.4 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 298 | 6%(w/v) PEG 4000, 0.2M ammonium acetate, 0.1M sodium citrate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
