3WSV
Crystal structure of minor L-lactate dehydrogenase from Enterococcus mundtii in the ligands-unbound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-26 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.430, 127.820, 133.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.960 - 2.380 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ldn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.083 | 0.409 |
Number of reflections | 71995 | |
<I/σ(I)> | 27.2 | 2.5 |
Completeness [%] | 99.5 | 96.3 |
Redundancy | 6.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 7% PEG 20000, 0.1M Mes-NaOH, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |