3WRW
Crystal structure of the N-terminal domain of resistance protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788, 0.9792, 0.9639 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.970, 105.280, 110.620 |
| Unit cell angles | 94.56, 109.27, 107.99 |
Refinement procedure
| Resolution | 49.640 - 2.710 |
| R-factor | 0.21059 |
| Rwork | 0.207 |
| R-free | 0.27781 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.543 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.640 | 2.860 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.237 | |
| Number of reflections | 79784 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 95.0 | 95.5 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 8.0% (W/v) PEG 8000, 0.4M ammonium tartrate dibasic, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
