3WRV
Crystal structure of NN domain of resistance protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.573, 101.396, 46.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.960 - 2.750 |
| R-factor | 0.19109 |
| Rwork | 0.188 |
| R-free | 0.24591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.960 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.273 | |
| Number of reflections | 9706 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 95.3 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 4% Tacsimate (v/v), 12% (w/v) PEG3500, pH 6.0, VAPOR DIFFUSION, temperature 293K |
