3WRG

The complex structure of HypBA1 with L-arabinose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Detector technology	CCD
Collection date	2013-10-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	77.586, 77.586, 254.176
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.230
Rwork	0.204
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.310
High resolution limit [Å]	2.230	2.230
Rmerge	0.050	0.447
Number of reflections	43124
<I/σ(I)>	36.2	4.4
Completeness [%]	97.9	98.3
Redundancy	5.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	295	0.4M ammonium acetate, 18%(w/v) polyethylene glycol 3350, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K