3WR7

Crystal Structure of Spermidine Acetyltransferase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 2 3
Unit cell lengths	148.688, 148.688, 148.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.020 - 2.500
R-factor	0.21566
Rwork	0.213
R-free	0.27595
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	2.049
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Rmerge	0.094
Number of reflections	38070
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	50mM sodium cacodylate pH 6.5, 9%(w/v) PEG 8000, 0.1M calcium acetate., VAPOR DIFFUSION, SITTING DROP, temperature 293K