3WR1
Crystal structure of Cormorant (Phalacrocorax carbo) hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.640, 55.640, 153.381 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.080 - 3.500 |
| R-factor | 0.19582 |
| Rwork | 0.188 |
| R-free | 0.26513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1faw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.080 | 13.080 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 2936 | |
| Completeness [%] | 77.2 | 77.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 25% PEG3350, 500mM NaCl, 10% Glycerol., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K |
