3WQ4
Crystal structure of beta-primeverosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-05-31 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54180 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.628, 88.796, 195.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.395 - 1.900 |
R-factor | 0.1612 |
Rwork | 0.159 |
R-free | 0.19470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cbg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.056 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.400 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.066 | 0.271 |
Number of reflections | 82106 | |
<I/σ(I)> | 4.3 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10mg/mL protein, 0.01% TritonX-100, 21% PEG 4000, 0.3M ammonium acetate, 0.1M sodium citrate (pH 5.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |