3WPU
Full-length beta-fructofuranosidase from Microbacterium saccharophilum K-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-31 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.242, 62.791, 83.569 |
| Unit cell angles | 88.74, 82.37, 72.63 |
Refinement procedure
| Resolution | 39.120 - 1.600 |
| R-factor | 0.16121 |
| Rwork | 0.160 |
| R-free | 0.19005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vsr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.287 |
| Number of reflections | 135214 | |
| <I/σ(I)> | 21.6 | 3.1 |
| Completeness [%] | 95.9 | 91.8 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM sodium acetate, 22.5% PEG 8000, 300mM Glycine, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
