3WPO

Acinetobacter sp. Tol 5 AtaA YDD-DALL3 domains in C-terminal stalk fused to GCN4 adaptors (CstalkC1i)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	159.750, 44.150, 95.730
Unit cell angles	90.00, 108.08, 90.00

Refinement procedure

Resolution	39.687 - 2.397
R-factor	0.2153
Rwork	0.212
R-free	0.27970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yo2
RMSD bond length	0.009
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.687		2.540
High resolution limit [Å]	2.397	7.090	2.400
Rmerge		0.032	0.735
Rmeas		0.038	0.861
Number of reflections	25148	1049	3859
<I/σ(I)>		27.52	1.91
Completeness [%]	98.8	98.2	95.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1M MES, 0.5M Ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K