3WPK
SPATIOTEMPORAL DEVELOPMENT of SOAKED PROTEIN CRYSTAL; 750 SEC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2013-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07345 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.175, 79.175, 37.886 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.17018 |
| Rwork | 0.169 |
| R-free | 0.19718 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2lym |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 8581 | |
| <I/σ(I)> | 22.6 | 5.6 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH METHOD | 4.5 | 293 | 25mg/ml Lysozyme, 0.05M SODIUM ACETATE, 0.7M SODIUM CHLORIDE, pH 4.5, BATCH METHOD, temperature 293K |
