3WPA
Acinetobacter sp. Tol 5 AtaA C-terminal stalk_FL fused to GCN4 adaptors (CstalkFL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 46.663, 46.663, 406.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.160 - 1.790 |
R-factor | 0.2037 |
Rwork | 0.203 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d9x 2yny 3wpo |
RMSD bond length | 0.006 |
RMSD bond angle | 1.048 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.790 | 3.880 | 1.800 |
Rmerge | 0.112 | 0.074 | 0.490 |
Number of reflections | 50426 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.6 | 97.9 | 98.7 |
Redundancy | 6.1 | 5.2 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 298 | 1.26M NaH2PO4, 0.14M K2HPO4, pH 5.1, vapor diffusion, hanging drop, temperature 298K |