3WPA
Acinetobacter sp. Tol 5 AtaA C-terminal stalk_FL fused to GCN4 adaptors (CstalkFL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 46.663, 46.663, 406.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.160 - 1.790 |
| R-factor | 0.2037 |
| Rwork | 0.203 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9x 2yny 3wpo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.048 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.790 | 3.880 | 1.800 |
| Rmerge | 0.112 | 0.074 | 0.490 |
| Number of reflections | 50426 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.6 | 97.9 | 98.7 |
| Redundancy | 6.1 | 5.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 298 | 1.26M NaH2PO4, 0.14M K2HPO4, pH 5.1, vapor diffusion, hanging drop, temperature 298K |
