3WP4
The crystal structure of native CDBFV from Neocallimastix patriciarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 64 |
Unit cell lengths | 92.770, 92.770, 42.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.800 - 1.270 |
R-factor | 0.14058 |
Rwork | 0.139 |
R-free | 0.16603 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1igo |
RMSD bond length | 0.013 |
RMSD bond angle | 1.649 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.320 |
High resolution limit [Å] | 1.270 | 1.270 |
Rmerge | 0.050 | 0.204 |
Number of reflections | 55625 | |
<I/σ(I)> | 49.4 | 7.9 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 8.8 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1M Tris, pH 8.5, 2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |