3WOW

Crystal structure of human CK2a with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-08
Detector	RAYONIX MX325HE
Wavelength(s)	0.90
Spacegroup name	P 21 21 21
Unit cell lengths	48.242, 78.460, 82.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.820 - 2.500
R-factor	0.1872
Rwork	0.184
R-free	0.23964
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3at2
RMSD bond length	0.016
RMSD bond angle	1.718
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.820
High resolution limit [Å]	2.500
Number of reflections	11362
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	25% ethylene glycol, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K