3WOV
Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (PaAglB-L, Q9V250_PYRAB, PAB2202) from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.748, 70.748, 204.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.616 - 3.110 |
| R-factor | 0.2322 |
| Rwork | 0.228 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.810 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 204.750 | |
| High resolution limit [Å] | 3.110 | |
| Rmerge | 0.073 | 0.948 |
| Number of reflections | 11310 | |
| <I/σ(I)> | 25.6 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.3 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% Jeffamine ED 2001, pH 7.0, 0.1M HEPES, 3% 6-aminohexanoic acid, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
