3WOU

Crystal Structure of The Recombinant Thaumatin II at 0.99 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-07-08
Detector	RIGAKU RAXIS V
Wavelength(s)	0.8
Spacegroup name	P 41 21 2
Unit cell lengths	57.685, 57.685, 150.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 0.990
Rwork	0.097
R-free	0.11850
Structure solution method	AB INITIO PHASING
RMSD bond length	0.018
RMSD bond angle	0.039
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.010
High resolution limit [Å]	0.990	0.990
Rmerge	0.069	0.496
Number of reflections	141082
<I/σ(I)>	64.34	6.26
Completeness [%]	99.8	100
Redundancy	8.9	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M ADA, 0.75M TARTRATE, 12.5% GLYCEROL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K