3WOO
Crystal structure of the DAP BII hexapeptide complex I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Detector technology | CCD |
| Collection date | 2013-02-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 121.700, 121.700, 218.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.920 - 1.800 |
| R-factor | 0.18441 |
| Rwork | 0.182 |
| R-free | 0.22436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3woi |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.881 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 143581 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 18% PEG 8000, 20% Glycerol, 2mM ZnCl2, 80mM CHES, 2mM Angiotensin II, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
