3WOJ

Crystal structure of the DAP BII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-01-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	76.460, 130.710, 170.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.590 - 2.200
R-factor	0.16773
Rwork	0.165
R-free	0.22003
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wok
RMSD bond length	0.019
RMSD bond angle	1.836
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Number of reflections	81815
Completeness [%]	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	18% PEG8000, 20% Glycerol, 2mM ZnCl2, 80mM CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K