3WOG
Crystal structure plant lectin in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.780, 195.481, 97.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.740 - 2.000 |
| R-factor | 0.2361 |
| Rwork | 0.235 |
| R-free | 0.26261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wcs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 49885 | |
| <I/σ(I)> | 36.6 | 6.6 |
| Completeness [%] | 99.0 | 98.1 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M bis-tris (pH 5.5), 2.0M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
