3WOE

Crystal structure of P23-45 gp39 (6-109) bound to Thermus thermophilus RNA polymerase beta-flap domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-24
Detector	RAYONIX MX225HE
Spacegroup name	P 41 21 2
Unit cell lengths	99.232, 99.232, 117.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.616 - 2.351
R-factor	0.1836
Rwork	0.181
R-free	0.22530
Structure solution method	MRSAD
RMSD bond length	0.008
RMSD bond angle	1.062
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	24886
<I/σ(I)>	19.8
Completeness [%]	99.5
Redundancy	17.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	293	50mM Tris-HCl (pH 8.0), 12% PEG 8000, 8% 2-propanol, 3% 1,6-hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 293K