3WOB
Crystal structure of a prostate-specific WGA16 glycoprotein lectin, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.880, 52.333, 45.300 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 44.110 - 2.600 |
| R-factor | 0.21712 |
| Rwork | 0.214 |
| R-free | 0.28317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aqg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.935 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.162 | 0.510 |
| Number of reflections | 3847 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.5M Sodium chloride, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 2%(v/v) Ethylene imine polymer, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
