3WO9

Crystal structure of the lamprey variable lymphocyte receptor C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	102.157, 37.229, 55.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.469 - 2.300
R-factor	0.1842
Rwork	0.181
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o6q
RMSD bond length	0.003
RMSD bond angle	0.762
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.113	0.322
Number of reflections	9798
<I/σ(I)>	11.6	4.1
Completeness [%]	100.0	92.8
Redundancy	6.9	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% PEG 3350, 0.2M potassium phosphate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K