3WO6
Crystal structure of YidC from Bacillus halodurans (form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-05 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.926, 60.631, 58.903 |
| Unit cell angles | 90.00, 100.35, 90.00 |
Refinement procedure
| Resolution | 28.297 - 2.403 |
| R-factor | 0.2429 |
| Rwork | 0.242 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeM-substituted protein structure |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.714 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX ((Phaser-MR)) |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.068 | 0.042 | 0.318 |
| Number of reflections | 10889 | ||
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 91.0 | 90.4 | 85.6 |
| Redundancy | 2.6 | 3.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipidic qubic phase | 6 | 293 | 28-32% PEG500DME, 2. mM CdCl2, 100mM Na-(CH3)2AsO2, pH 6.0, lipidic qubic phase, temperature 293K |
