3WN8
Crystal Structure of Collagen-Model Peptide, (POG)3-PRG-(POG)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-21 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.285, 19.567, 49.022 |
| Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.450 |
| Rwork | 0.141 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.023 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.450 |
| Rmerge | 0.062 |
| Number of reflections | 7224 |
| Completeness [%] | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 15 % PEG 4000, 0.05M Tris-HCl, 0.005M Lithium Sulfate, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
