3WN6
Crystal structure of alpha-amylase AmyI-1 from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.917, 125.277, 96.642 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 48.320 - 2.160 |
| R-factor | 0.20161 |
| Rwork | 0.199 |
| R-free | 0.24323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bg9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.469 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.099 | 0.240 |
| Number of reflections | 97842 | |
| <I/σ(I)> | 22.6 | 5.9 |
| Completeness [%] | 98.4 | 89 |
| Redundancy | 5.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 2% tacsimate, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
